Extended Data Fig. 2: Adenine base-editing efficiency by different gRNAs.
From: High-efficiency base editing in the retina in primates and human tissues
A) Schematic of a fragment of the ABCA4 exon 42 with the NGG-PAM sequence indicated in purple, the Stargardt c.5882A target base highlighted in red, and the c.5883A wobble base shown in blue. B) Base-editing efficiencies at the target- and wobble bases with the unsplit ABE7.10 base editor in combination with the different gRNAs in lenti-ABCA41961E HEK293T cells. Results were obtained from three biological replicates (eyes) and are presented as mean ± s.d. *P < 0.05, ***P < 0.001 by three-way mixed-effect ANOVA with Tukey’s correction. Illustration in b created with BioRender.com.
