Extended Data Fig. 1: Identification of circulating tumor cells.
From: Neoadjuvant triplet immune checkpoint blockade in newly diagnosed glioblastoma
Representative region of whole-slide immunofluorescence showing circulating tumor cell (CTC, boxed) identified in a GBM patient. Cells were stained for GFAP (green), EGFR (yellow), CD45/CD66b (white) and nuclear DAPI (blue). Magnification, 40X. Sample collected pretreatment (day –9). 7.5 mL peripheral blood was processed on the AccuCyte-CyteFinder platform (RareCyte). Nucleated blood cells were spread on 8 slides. Slides were stained using DAKO autostainer and the whole slide was imaged and analyzed by CyteMapper software for DAPI + GFAP + EGFR + /− CD45/CD66b− cells with user verification. Scale bars = 100 μm.
