Fig. 4: Immune cell subcluster in the PCOS-endometrium and effect of metformin and lifestyle.
From: Single-cell profiling of the human endometrium in polycystic ovary syndrome
a, UMAP of subclustered immune cells (n = 13,596) from all individuals (n = 27). b, Heatmap of scaled log(transformed) gene expression characterizing 15 immune subclusters. c, Bar plot showing the proportion of the 15 immune subclusters from control (n = 5), PCOS (n = 12), PCOS-metformin (n = 7) and PCOS-lifestyle (n = 3) groups. d, Total number of DEGs in the six immune subclusters and dotplots of the top five up- and downregulated DEGs (control versus PCOS) in these immune subclusters. e, GO enrichment analyses on DEGs split on up- or downregulation, within six immune subclusters at baseline between PCOS and controls, and between PCOS-baseline and after 16 weeks of intervention with metformin or lifestyle, respectively. GOs with BH-adjusted P < 0.05 are reported and −log10(transformed) for visualization. f, Heatmap showing DEGs in GOs between controls and cases of PCOS and those that are reversed by metformin or lifestyle intervention. g, UMAP of subclustered endothelial or lymphatic cells from all individuals. h, Bar plot showing the proportion of the five endothelial or lymphatic subclusters from control (n = 5), PCOS (n = 12), PCOS-metformin (n = 7) and PCOS-lifestyle (n = 3) cases. i, Heatmap of scaled log(transformed) gene expression characterizing the five endothelial or lymphatic subclusters. j, Violin plot showing DEGs in GOs between PCOS cases and controls, and between PCOS-baseline and after 16 weeks of intervention with metformin or lifestyle, respectively. The line inside the box indicates the median value and the box delimits the 25th and 75th percentiles. The smallest and largest values are indicated by the lines outside the box. k, GO enrichment analyses on DEGs split on up- or downregulation, within endothelial artery and lymphatic subclusters at baseline between control and PCOS cases and between PCOS-baseline and after 16 weeks of intervention with metformin or lifestyle, respectively. GOs with BH FDR P < 0.05 are reported and −log10(transformed) for visualization. See ‘Statistical analyses’ for a detailed description of DEG analysis and GO enrichment analysis statistics.
