Extended Data Fig. 1: snRNA-seq individual samples and quality control.
From: Single-cell profiling of the human endometrium in polycystic ovary syndrome
a) Uniform Manifold Approximation and Projections (UMAP) of 27 integrated samples, showing sample ID’s, grouping, cell cycle phase and UMAP clusters. b) UMAP projections of integrated samples and gene expression of the major cell type markers EPCAM for epithelium, IGF1 for stroma, GUCY1A2 for uterine smooth muscle cells, CD14 for myeloid cells, NCAM1 for lymphoid celss, VWF for endothelium and PROX1 for lymphatic cells. c) UMAP projection of major cell types including undefined nuclei. d) Bar plot of the nuclei proportions of the main cell types in the 27 samples. e) Fluorescence microscopy control images of one PCOS and one control endometrial tissue for estrogen receptor alpha (Fig. 2f) using Rabbit IgG Isotype Control antibody. f) Uniform Manifold Approximation and Projection (UMAP) projections of integrated snRNA-seq data from 5 women with PCOS and Provera-induced bleeding and 7 women with PCOS with non-induced bleeding showing seven cell type-specific clusters. g) Bar plot of the nuclei proportion of major cell types of 5 samples with Provera-induced bleeding and 7 with non-induced bleeding.
