Extended Data Fig. 4: Profiling of apMCs in patient-derived breast tumors. | Nature Medicine

Extended Data Fig. 4: Profiling of apMCs in patient-derived breast tumors.

From: Mobilizing antigen-presenting mast cells in anti-PD-1-refractory triple-negative breast cancer: a phase 2 trial

Extended Data Fig. 4

(a, b) Panel design (a) and association of apMC infiltration with other cells in tumor microenvironment (b, n = 9) using MIBI-TOF. Tryptase+HLA-DR+ cells were identified as apMCs. (c) Correlation of multiplex immunohistochemistry versus RNA-seq quantified apMC level (n = 186). (d) Receiver operating characteristic (ROC) curve and area under curve (AUC) of the apMC signature level for staining-confirmed apMC infiltration. (e) Correlation between macrophage (stained by CD68) and apMC infiltration in TNBC. (f) Scaled deconvolution values for apMC and TLS overlay onto tissue spots using spatially resolved transcriptomics. (g) Spatial pattern of apMC and TLS localization in TNBC. Data are mean ± s.e.m. (b) and error bands of the line plot indicate 95% CI (g). Two-sided unpaired Student’s t-test (b), Spearman’s correlation method (c, e).

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