Extended Data Fig. 3: Safusidenib impact on tumor microenvironment composition.

a. UMAP overlaid with participant origin for each nucleus. b. UMAP colored by UMI count (log₁₀ transformed). c. UMAP colored by number of detected genes (log₁₀ transformed). d. UMAP of snRNA-seq data colored by closest cell annotation match in Spitzer et al.²⁹ predicted using SingleR. e. Confusion matrix of proposed cell annotation versus predicted Spitzer at al. cell annotation. f. Heatmap of z-score of average expression of well-established markers in different cell types. g. Bar plot of proportion of cell types pre- and post-safusidenib for each participant from snRNA-seq data. h. Difference in proportion of all major cell types between snRNA-seq samples pre- and post-safusidenib. i. Difference in proportion of tumor cell types between snRNA-seq samples pre- and post-safusidenib. j. Difference in proportion of immune cell types between snRNA-seq samples pre- and post-safusidenib.