Supplementary Figure 4: Qualitative iSIM comparisons highlighting the effect of TIRF.
From: Single-shot super-resolution total internal reflection fluorescence microscopy
U2OS cells were fixed, microtubules immunostained with Alexa Fluor 488, and the same field imaged in instant SIM (non-TIRF illumination) (a) and instant TIRF-SIM (b). Arrows in higher magnification views (c, d) highlight features evident in instant SIM imaging but absent or attenuated in instant TIRF-SIM. Images have been delined as descripted in Supplementary Fig. 5–7. Scale bars: 10 μm in a, b; 2 μm in c, d. Experiments were repeated at least 4 times with similar results; representative data are shown.
