Supplementary Figure 6: Residual intensity variations after the delining procedure.
From: Single-shot super-resolution total internal reflection fluorescence microscopy
a) Fluorescent dye, imaged in instant TIRF-SIM, flat-fielded and delined according to procedure in Supplementary Fig. 5. Red and blue lines indicate profiles in vertical (perpendicular to line artifact) and horizontal (parallel) directions. b) Variation in intensity along line profiles in a). Although vertical variations are larger than horizontal, they are still only ~2.4% of the mean intensity of the sample. Scale bar: 5 μm. Experiments were repeated 3 times with similar results ; representative data are shown.
