Supplementary Figure 3: Experimental PSF with a low number of photons and Cramèr–Rao lower bound calculated with background noise. | Nature Methods

Supplementary Figure 3: Experimental PSF with a low number of photons and Cramèr–Rao lower bound calculated with background noise.

From: Self-interference 3D super-resolution microscopy for deep tissue investigations

Supplementary Figure 3

(a) (first row) Measured interferograms of a 100 nm fluorescent bead (tetraspeck) in-focus (z = 0) or defocused within the depth-of-field (z = ±200, ±400 nm) with approximately 1000 photons per PSF. (second row) Numerical Fourier Transform of the interferograms. (b) CRLB considering a photon background of b = 1000 photons/µm².

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