Supplementary Figure 5: MiniLFM–SID volumetric Ca2+ imaging up to 260 µm deep in hippocampus CA1 of a freely moving mouse expressing nucleus-confined Ca2+ indicator. | Nature Methods

Supplementary Figure 5: MiniLFM–SID volumetric Ca2+ imaging up to 260 µm deep in hippocampus CA1 of a freely moving mouse expressing nucleus-confined Ca2+ indicator.

From: High-speed volumetric imaging of neuronal activity in freely moving rodents

Supplementary Figure 5

(a) Neuron positions obtained by SID-analysis of an 8-minute MiniLFM recording at 16 Hz frame rate in mouse hippocampus expressing nucleus-localized indicator AAV9.Syn.H2B.GCaMP6f.WPRE.Pzac2.1 (top and side views). (b) Heatmap of motion-corrected and de-noised temporal signals corresponding to the 127 neuron positions shown in (a). (c) Stacked neuronal activity traces, same data as in (b). (d) Histogram of the neuron positions shown in (a) versus depth. (e) Histogram of nearest-neighbor distances between the neurons shown in (a).

Back to article page