Supplementary Figure 1: Conditionally expressed proteins regain metabolic regulation when tagged in a native promoter/regulation manner.
From: Genome-wide SWAp-Tag yeast libraries for proteome exploration
(a) NOP1pr-GFP-GAL2 yeast strain before and after native promoter/regulation GFP swap to a NATIVEpr-GFP tag in synthetic medium with either 2% glucose or 2% galactose. (b) NOP1pr-GFP-PHO5 yeast strain before and after native promoter/regulation GFP swap to a NATIVEpr-GFP tag in synthetic medium with or depleted of phosphate. (c) NOP1pr-GFP-SUC2 yeast strain before and after native promoter/regulation GFP swap to a NATIVEpr-GFP tag in synthetic medium with either 2% or 0.2% glucose. All scale bars are 5µm. Imaging of strains was performed a single time. Images represent entire field.
