Supplementary Figure 3: N′ SWAT tagging of proteins bearing an MTS.
From: Genome-wide SWAp-Tag yeast libraries for proteome exploration
(a) Two MTS-containing proteins, Tuf1 and Coq10, were N’-tagged with and without a generic MTS in the tagging cassette. (b) Native promoter/regulation swapping of N’ SWAT-tagged Tuf1 restores the native MTS targeting to mitochondria. All scale bars are 5µm. Imaging of strains was performed a single time. Images represent entire field. (c) Mitochondrial proteins tagged with a TEF2pr-mCherry without an MTS (d) MTS and TMD prediction analysis for proteins showing mitochondrial localization with the NOP1pr-GFP, NATIVEpr-GFP, and/or C’ GFP tags. Cx(9)C - cysteine-rich domain26, β-barrel domain27. All scale bars are 5µm. Imaging of strains was performed a single time. Images represent entire field.
