Supplementary Figure 4: Ysa1 is dual-localized to mitochondria.
From: Genome-wide SWAp-Tag yeast libraries for proteome exploration
(a) N’-tagged Ysa1 under its native promoter has a fraction that co-localizes with the mitochondrial marker Tom20-mCherry. (b) C’-tagged Ysa1 has a fraction that co-localizes with the mitochondrial marker Tom20-mCherry. (c) α-tagged Ysa1 is dually localized between mitochondria (M) and cytosol (C) in subcellular fractionation. Total lysate (T) is shown as control. Hsp60 is probed as a mitochondrial protein. Hxk1 is probed as a cytosolic protein. All scale bars are 5µm. Imaging of strains was performed a single time. Images represent entire field.
