Supplementary Figure 5: Repression of endogenous genes with the top tripartite fusions compared with that observed with the bipartite KRAB–MeCP2 repressor.
From: An enhanced CRISPR repressor for targeted mammalian gene regulation
The three best performing tripartite repressors based on the reporter screen were selected and tested against a series of endogenous loci, along with HP1A-KRAB and KRAB-MeCP2. RNA levels of the four indicated genes from a multiplexed repression experiment were measured using RT-qPCR. n = 2 biologically independent samples (cell cultures).
