Supplementary Figure 6: Reporter screen of different dCas9 fusion proteins containing KRAB or MeCP2.
From: An enhanced CRISPR repressor for targeted mammalian gene regulation
(a) Schematic of CRP repression device. dCas9 repressors coupled with a U6-driven gRNA regulate EYFP output through binding two targets sites within the CRP promoter. (b) dCas9-KRAB-MeCP2 outperformed either KRAB or MeCP2 either as single or double fusions to dCas9 when paired with sgRNA in repressing EYFP output. Data are presented as mean ± s.e.m. n = 3 biologically independent samples (cell cultures). One-sided Student’s T-test was used for statistical comparison. ¥ indicates p < 0.05 vs. dCas9, * indicates p < 0.05 vs. dCas9-KRAB.
