Supplementary Figure 9: Cluster analysis of the top 35 DE genes in negative control, dCas9, dCas9–KRAB, and dCas9–KRAB–MeCP2 groups.
From: An enhanced CRISPR repressor for targeted mammalian gene regulation
a) Clustering of genes with correlated expression provides insights into the biological effects of repressor’s activity. Heatmap representing distances between each gene pair is calculated based on Euclidean distance, (1-R)2/2 where R represents the Pearson’s correlation of two genes. A scale key bar of normalized log2 CPM shows large negative (colored in blue) and positive (colored in red) correlations. Genes with large positive correlations correspond to small Euclidean distances and cluster together. (b-c) Shown are Venn diagrams comparing transcriptome-wide downregulated or upregulated genes among the different repressors. (Methods associated with this figure are described in Supplementary Note 2).
