Supplementary Figure 10: HAP1 cells stably expressing dCas9 repressors.
From: An enhanced CRISPR repressor for targeted mammalian gene regulation
(a) Shown is the expression level of dCas9 repressors in the different stable cell lines. n = 2 biologically independent samples (cell cultures). (b) Stable cells were transduced with lentiviruses containing a gRNA targeting the indicated genes. dCas9-KRAB-MeCP2-containing cell line induced stronger suppression of most target genes. For a-b, n = 2 biologically independent samples (cell cultures). (c) Shown are distributions of sgRNA constructs transduced into HAP1 wild-type cells over time. The sgRNA counts remained similar to that of the initial DNA library used to generate lentivirus. Blue squares represent non-essential gene-targeting constructs and orange squares represent essential gene-targeting constructs.
