Supplementary Figure 14: Characterized adaptive astigmatism amplitude and apparent focal shift.

(A) The magnitude of astigmatism as a function of depth to achieve the depth-independent calibration curve(s) in Fig. 1K and Supplementary Figure 5C is represented by the solid line, while the dashed black line shows the constant astigmatism used in Supplementary Figure 5A,B. (B) The apparent focal position of the beads with and without adaptive optics correction were carefully measured to determine the apparent focal shift due to the index mismatch.