Supplementary Figure 15: Histograms of localization precisions.

(A) Histogram of the averaged lateral localization precisions calculated from CRLB described in Methods based on background, axial position and photon emitted from single molecules assuming emitter locates at the center of the sub-region for Fig. 1N. (B) The axial localization precisions as calculated as described in Methods based on background, axial position and photon emitted from each localized single molecule for Fig. 1N. The calculations for (C) lateral and (D) axial localizations precisions for Fig. 2. Note that these axial precision measures correspond well (after converting into FWHM values) with FWHM of line profiles through thin filaments shown in Supplementary Figure 9.