Supplementary Figure 1: Lateral localization precision at different depths. | Nature Methods

Supplementary Figure 1: Lateral localization precision at different depths.

From: Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections

Supplementary Figure 1

Average lateral localization precisions at the focal plane as a function of bead depth for nine different beads, quantified using Cramér–Rao lower bound (CRLB, Methods). The PSFs without AO correction suffer from increased blurring due to depth as well as reduced numbers of photons. AO correction improves the quality of the PSF and the number of photons collected. The inclusion of Adaptive Astigmatism (AA) spreads the PSF signal out compared to the AO only PSFs resulting in a slightly (~20%) worse lateral localization precision. However, this slight loss in lateral precision is acceptable compared to the significant gain in axial localization precision (Fig. 1L).

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