Supplementary Figure 2: Zernike mode corrections for the data in Figs. 1 and 2. | Nature Methods

Supplementary Figure 2: Zernike mode corrections for the data in Figs. 1 and 2.

From: Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections

Supplementary Figure 2

Corrected Zernike mode amplitudes (A.U.) for (A) PSFs in Fig. 1, (B) COS-7 cell labeled with TOM20-Alexa 647 in Fig. 1N and (C) beta-amyloid plaque image in Fig. 2. Zernike Mode numbers correspond to the Noll indices; Oblique Astigmatism (Z5), Vertical Astigmatism (Z6), Horizontal Coma (Z7), Vertical Coma (Z8), 1st order Spherical Aberration (Z11), and 2nd order Spherical Aberration (Z22). The data in A is representative of eight repeated experiments. The data in B is representative of three imaged cells and the data in C is representative of 37 imaged plaques.

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