Supplementary Figure 6: Immunoglobulin gene repertoire of human class-switched memory B cells in HIS mice.

(a) Circular phylograms of representative clonal expansions as shown in B (green and blue clones for BRGS and BRGST, respectively). GL, germline; LN, lymph nodes; Sp, spleen; BM, bone marrow. (b) Circos plots comparing the frequency of VH(DH)JH rearrangements of IgA⁺/IgG⁺ memory B-cell antibodies between both groups. (c) Violin plots comparing the number of mutations in the variable genes of heavy (IgH) chain between both groups. The colored bar and the black dot indicate mean and median, respectively. Below each dot plot, pie charts show the distribution of mutation in each group, from 0 (white) to 7 or more (red). The number of sequences analyzed (n) in each group is indicated below the Violon plot. Groups were compared using unpaired two-sided student t-test with Welch’s correction. LN, lymph nodes; Sp, spleen; BM, bone marrow. (d) Graphs show the Bayesian estimation of antigen-driven selection in IgH CDR sequences from both groups as determined with the BASELINe software using a total of 121 and 115 IgL sequences for BRGS and BRGST, respectively (following the groups distribution shown in (c)). Values >0 indicate positive selection.