Supplementary Figure 1: Mouse ILC and LN anlage development in BRGST mice.
From: A human immune system mouse model with robust lymph node development
(a) Mouse TSLP concentration in plasma from BRGS and BRGST mice was quantified by ELISA (n = 12 mice/group, bars represent mean, and error bars denote s.e.m.). (b) Representative FACS plot showing the gating strategy for mouse ILCs in lamina propria of small intestines of BRGST mice from 12 mice with similar results. (c) Absolute numbers of ILC subsets in small intestines of Rag2−/−, BRGS and BRGST mice (n = 4 mice/group; bars represent mean, and error bars denote s.e.m., P value is shown, one-way ANOVA with a Turkey test) (d) Representative images of anlagen of inguinal and iliac LNs in non-engrafted BRGS and BRGST mice 1 week after peritoneal injection of Chicago Sky Blue 6B dye from 2 independent experiments with similar results. LN anlagen are indicated by arrow heads. Scale bar = 2 mm. (e) Total number of LN anlagen observed in non-engrafted 8 week-old BRGS and BRGST littermates 1 week after dye injection (n = 5 mice/group, bars represent mean, and error bars denote s.e.m., P value is shown for Two-tailed Mann-Whitney U test). (f) Quantification of different LNs present in non-engrafted 8 week-old BRGS and BRGST littermates (n = 8 mice/group) 1 week after dye injection. To calculate the percentages, the number of mice observed with indicated LN anlage was divided by total number of mice from indicated strain. (g) Numbers of PPs counted on small intestines of BRGST and C57BL/6 newborn pups after VCAM1 whole mount staining (n = 4 mice/group, bars represent mean, and error bars denote s.e.m.).
