Supplementary Figure 3: Two-photon microscopy of explanted BRGST LN showing human T cell behavior.
From: A human immune system mouse model with robust lymph node development
10 million human T cells purified from health donor PBMC were fluorescently labeled and adoptively transferred into 12 week old BRGST HIS mouse. After 24 h, intact inguinal lymph nodes were explanted and subjected to two-photon imaging (a). Representative images of human T cells and corresponding tracks from 8 different movies in 3 different recipients. Scale bar = 20 µm. Right side insets show the migration pattern for single human T cells (n = 3). Scale bar = 10 µm. Graphs show the mean velocity (b) and the straightness index (c) for individual T cells (the measure of centre represents mean). (d) T cells tracks were graphed from a common origin. Data are representative of 8 different movies in 3 different recipients.
