Fig. 1: Schematic of the FRET standard molecules.
From: Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
Double-stranded DNA was labeled with a FRET pair at 15-bp or 23-bp separation for the “lo” and “mid” samples, respectively (sequences are provided in the Methods). The accessible volumes (AVs) of the dyes (donor, blue; acceptor, red) are illustrated as semi-transparent surfaces and were calculated with freely available software8. The mean dye positions are indicated by darker spheres (assuming homogeneously distributed dye positions; Supplementary Note 3). The distance between the mean dye positions is defined as RMP,model. Calculated values for RMP,model and the errors obtained by varying parameters of the AV model are shown (Supplementary Note 3). The B-DNA model was generated with Nucleic Acid Builder version 04/17/2017 for Amber27.
