Fig. 4: Mean interdye distances determined from 19 〈E〉 values measured in 16 different labs.
From: Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
a,b, R〈E〉 for samples 1 (a) and 2 (b). c,d, RMP for samples 1 (c) and 2 (d). Data are shown as individual values (colored symbols) and as the mean (black dots) and s.d., assuming R0 = 62.6 Å and R0 = 68.0 Å for samples 1 and 2, respectively. The black bars at the top of each plot indicate the static model values and their error (determined by variation of model parameters); see Supplementary Table 4 for values. The depicted errors include only the statistical variations of the FRET efficiencies, and do not include the error in the Förster radii; thus these errors represent the precision of the measurement, but not the accuracy. Exp., experimental.
