Cas9 induces larger-than-anticipated mutations in mouse and human cells. In the latter, efficient editing depends on inhibition of the DNA-damage-repair protein p53.
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Research papers
Kosicki, M. et al. Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements. Nat. Biotechnol. https://doi.org/10.1038/nbt.4192 (2018).
Haapaniemi, E. et al. CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response. Nat. Med. 24, 927–930 (2018).
Ihry, R. J. et al. p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells. Nat. Med. 24, 939–946 (2018).
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Rusk, N. Surprising CRISPR roadblocks. Nat Methods 15, 569 (2018). https://doi.org/10.1038/s41592-018-0097-9
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DOI: https://doi.org/10.1038/s41592-018-0097-9
