Supplementary Figure 7: Validation of potential MUL1-interacting proteins.
From: A proximity-tagging system to identify membrane protein–protein interactions
(a) Pathway analysis of MUL1 interaction proteins. Each dot represents a protein identified in Supplementary Table 4 as a MUL1 specific interacting protein. These proteins are either connected in primary metabolic process or related to ubiquitin-proteasome system. The gene ontology software STRING (functional protein association networks) was used to analyze protein interactions (https://string-db.org/). The thickness of the lines indicates the strength of data support. (b) Co-immunoprecipitation of MUL1 and interacting candidates. Myc tagged MUL1 was co-transfected with different V5 tagged candidate in HeLa cells. LCK-V5 was used as the negative control. MUL1 associates with TOMM22, CYB5R1 and PEX19, but not with OCIAD1 and RHOT1. (c) TOMM22 and PEX19 interact with MUL1. The co-immunoprecipitation experiments were repeated with TOMM22 and PEX19 to further confirm the specific interaction. Experiments in (b) and (c) were repeated independently twice with similar results. Original full scans of blots are shown in Supplementary Fig. 15.
