Supplementary Figure 10: Recombinant protein expression and purification.
From: A proximity-tagging system to identify membrane protein–protein interactions
(a) Construct design and protein sequence used to express and purify IL2-FKBP. Human IL2-FKBP fusion with GS linker and His tag was subcloned into pCDNA3.1 and transfected 293 T cells. Medium containing IL2-FKBP was collected and the protein was purified with Ni beads. FRB-PafA was subcloned into pGEX6p-1 and fused with N terminal GST (see Methods for details). (b) Purified IL2-FKBP and (c) Purified FRB-PafA are analyzed with SDS-PAGE and coomassie staining. Lane 1 in (c) is purified GST-FRB-PafA cleaved by PreScission protease and lane 2 is further purified FRB-PafA.
