Supplementary Figure 11: Histogram of the overall registration error (rotation angle) for the simulated 100% DOL dataset, which included 256 TUD logos.
From: Template-free 2D particle fusion in localization microscopy
A normal distribution with a standard deviation of 0.9 degree (red curve) is shown for comparison. Since the length of the bounding box of the logo is 70 nm, this amount of error will maximally result in a displacement of ±0.55 nm at the edges of the logo. This is smaller than the distance of the binding sites (5 nm) and very close to the mean localization uncertainty. Consequently, the impact of the overall outlier removal is minimal on the final reconstruction as depicted in Supplementary Fig. 2g.
