Supplementary Figure 6: Comparison of particle-fusion performance by our method and EMAN.2 on simulated STORM datasets as a function of DOL (bleaching rate corresponding to an average of ~33 localizations per binding site and 5,000 photons per localization event).

(a-c) Ground-truth fusion of 256 particles (d-f) Our reconstructions. (g-i) Reconstructions using EMAN.2 with 71, 90 and 128 included particles (minimum of three classes for the class averaging). With the chosen bleaching rate, the average number of localizations per particle for the three datasets is similar to the corresponding simulated PAINT data in Supplementary Fig. 2. While for 100% DOL the reconstruction of STORM data is as good as for PAINT and close to the ground-truth, successful reconstructions require a DOL of at least ~50%. Our method outperforms EMAN.2 for all degrees of underlabeling.