Supplementary Figure 8: Simultaneous imaging of spine-jRGECO1a and QuasAr2.
From: All-optical synaptic electrophysiology probes mechanism of ketamine-induced disinhibition
a, Co-expression of spine-jRGECO1a and QuasAr2. Schematic showing three color imaging with blue light excitable soma-localized CheRiff in presynaptic cells, yellow light excitable spine-jRGECO1a and red light excitable QuasAr2 co-expressed in postsynaptic cells. b, Dual-view microscope for simultaneous imaging of spine-jRGECO1a and QuasAr2. Fluorescence from spine-jRGECO1a and QuasAr2 were passed to electron-multiplying charge-coupled-device camera and sCMOS camera, respectively, by a 640 nm dichroic beamsplitter.
