Supplementary Figure 8: Additional validated hits from the HSPC screen.
From: Guide Swap enables genome-scale pooled CRISPR–Cas9 screening in human primary cells
(a) Cumulative distribution of the Log2 normalized reads per million per gRNA in the CD34- (blue) and CD34 + (magenta) populations. (b) Representative FACS plots of CD45RA and CD34 expression 10 days post-electroporation with indicated RNPs; two technical replicates in one experiment. (c) Representative FACS plots of fluorescence in the APC channel and CD34 expression 10 days post-electroporation with indicated RNPs; two technical replicates in one experiment.
