Supplementary Figure 6: Pooled Guide Swap screens in human primary CD4+ T cells.
From: Guide Swap enables genome-scale pooled CRISPR–Cas9 screening in human primary cells
(a) Cumulative distribution of the Log2 normalized reads per million per gRNA at Day 0 (magenta) and Day 6 RFP + CD4 + CD45 + CXCR4 + (blue) for one replicate. (b-c) Comparison of normalized reads per million in (b) Day 0 replicates and (c) Day 6 RFP + CD4 + CD45 + CXCR4 + replicates. r = Pearson correlation coefficient calculated from two technical replicates. (d) Day 6 RFP + CD4- versus Day 0 comparison of log2 fold changes in replicates for gRNAs that regulate CD4 surface expression. CD4 spike-in gRNAs are colored in blue. CD4 gRNAs present in the library are colored in magenta. (e) Day 6 RFP + CD45- versus Day 0 comparison of log2 fold changes in replicates for gRNAs that regulate CD45 surface expression. CD45 spike-in gRNAs are colored in blue. CD45 gRNAs present in the library are colored in magenta. Two gRNAs that were spiked in were also present in the library, and these are colored in purple. (f) Venn diagram of gene hits from RSA and 2nd best gRNA analyses of Day 6 RFP + CD4 + CD45 + CXCR4 + versus Day 0 samples.
