Supplementary Figure 1: Automatic map segmentation.
From: A fully automatic method yielding initial models from high-resolution cryo-electron microscopy maps
A. Automatic segmentation of map for β-galactosidase1 at a resolution of 2.2 Å (EMD entry 2984). Symmetry was identified using the Phenix tool phenix.map_symmetry and specifying the symmetry as D2. The map, the sequence of β-galactosidase and the symmetry file were supplied as inputs to the Phenix tool phenix.map_box with the keyword extract_unique=True. The automatically extracted unique part of the map is shown in red and the entire map is shown in yellow. B. Automatic segmentation of map for group II chaperonin2 at a resolution of 4.3 Å (EMD entry 5137). The map, the sequence and symmetry (D8) were supplied as inputs to the Phenix tool phenix.map_box along with the keyword extract_unique=True. The automatically extracted unique part of the map is shown in red and chains A (green) and H (blue) of the deposited model (3Los) are shown. Graphics created with Chimera3. References: 1. Bartesaghi, A. et al. Science 348, 1147–1151 (2015). 2. Zhang, J. et al. Nature 463, 379–383 (2010). 3. Pettersen, E.F. et al. J. Comput. Chem. 25, 1605–1612 (2004).
