Supplementary Figure 4: RNA model building. | Nature Methods

Supplementary Figure 4: RNA model building.

From: A fully automatic method yielding initial models from high-resolution cryo-electron microscopy maps

Supplementary Figure 4

RNA model-building into a cut-out region of RNA density from Leishmania ribosome1 at a resolution of 2.5 Å (EMDB entry 7025). A. Model building and real-space refinement using base-paired RNA helices. B. Model building and real-space refinement with the same map using standard nucleic acid model building. Potential hydrogen bonds indicated by dotted red lines. Graphics created with Chimera2. References: 1. Shalev-Benami, M. et al. Nat. Commun. 8, 1589 (2017). 2. Pettersen, E.F. et al. J. Comput. Chem. 25, 1605–1612 (2004).

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