Supplementary Figure 5: Comparison of osmFISH and scRNA-seq. | Nature Methods

Supplementary Figure 5: Comparison of osmFISH and scRNA-seq.

From: Spatial organization of the somatosensory cortex revealed by osmFISH

Supplementary Figure 5

(a) Comparison between the distribution of counts for osmFISH (blue, left) and scRNA-seq (red, right). (b) Comparison between the 99th percentile of the osmFISH and scRNA-seq data. osmFISH shows higher counts compared to scRNA-seq, except for a few genes which are characterized by high expression level that saturates the optical space. On average osmFISH shows 3.8 ± 4.2 times higher measurements than scRNA-seq. (c) Cell type correlation between mean expression levels for osmFISH and scRNA-seq clusters (Zeisel et al.16), n = 33 genes. Highest correlation (⍴ = 1) is visualized in yellow.

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