Supplementary Figure 10: HUMAnN2 compared with other methods: isolates of novel species, UniRef50-based COG gold standard.
From: Species-level functional profiling of metagenomes and metatranscriptomes
This figure mirrors Supplementary Fig. 8, except that COG annotations are defined based on co-clustering with UniRef50 families (rather than UniRef90). Similarly, HUMAnN2 was run in UniRef50 mode. These changes tend to favor sensitivity over specificity during both isolate genome annotation and profiling. (a) Accuracy and performance of the six functional profiling methods. (b) Observed versus expected COG abundance
