Supplementary Figure 3: LM image data overlaid on top of the low-resolution EM data after affine LM–EM alignment. | Nature Methods

Supplementary Figure 3: LM image data overlaid on top of the low-resolution EM data after affine LM–EM alignment.

From: Nanobody immunostaining for correlated light and electron microscopy with preservation of ultrastructure

Supplementary Figure 3

The corresponding video (Supplementary Video 3) demonstrates the alignment precision reached by a single linear (3D affine) transformation across an example section of the dataset and how to use LM to assist cell-type identification in EM data. This is a screen capture from the VAST 2D view

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