Supplementary Figure 3: Cas9 inhibition is dose dependent.
From: Engineered anti-CRISPR proteins for optogenetic control of CRISPR–Cas9
HEK293T cells were co-transfected with plasmids encoding (i) Acr–LOV hybrid, (ii) Cas9 and (iii) a luciferase reporter as well as a gRNA targeting the luciferase gene. The vector mass ratio of the transfected Cas9 and Acr–LOV construct was varied between 10:1 and 1:1, as indicated. Six hours post-transfection, cells were irradiated with pulsatile blue light (5 s ON, 10 s OFF; 2.5 W per m2) for 30 h or kept in the dark as control before assessing luciferase activity. Box plots show the median (center line) and first and third quartiles (box edges), 1.5× the interquartile range (whiskers) and individual data points (circles). n = 9 biologically independent samples (cell cultures) for the Acr–LOV hybrid 8 (1:1) and n = 12 biologically independent samples (cell cultures) for all other conditions.
