Supplementary Figure 14: Assessing long-term effects of photostimulation.
From: Closed-loop all-optical interrogation of neural circuits in vivo
(a) The percentage of cells exhibiting nuclear fluorescence is 4.2 ± 2.6% on day 1 (before running the conditioning protocol in Fig. 2), and 4.0 ± 2.4% on day 2 (24–48 h after running the plasticity protocol; 49 cells in 5 FOVs in 5 animals, n.s. P = 1.0, Wilcoxon signed-rank test (two-sided); measure of center is mean; error bars show s.d.). (b) The photostim-triggered ΔF/F of cells in (a) (average amplitude of 10 trials, 4 mW per cell, 15 10-ms spirals at 100 Hz, Satsuma laser) on day 2 is similar to the levels on day 1 (n.s. P = 0.69, Wilcoxon signed-rank test (two-sided); measure of center is mean; error bars show s.d.). (c) The GCaMP6f image, photostimulation-triggered ΔF and the C1V1-mCherry images of the tested cells on day 1 and day 2. The cells marked in red squares are identified as ‘filled’ according to their GCaMP6f intensity (left panel). Note that many of them still showed visible GCaMP responses when activated by photostimulation (middle panel).
