Supplementary Figure 15: Quantification of motion-induced artifacts.
From: Closed-loop all-optical interrogation of neural circuits in vivo
(a) During the 30-min closed-loop conditioning in Fig. 2 (animals were head-fixed and were freely running on a running wheel), the lateral shifts of the fields of view are within 5 μm in 99% of all frames (324,000 frames in 6 movies). (b) The average ΔF/F recorded from the trigger cell in the raw movies (without motion correction) is close to that extracted from the registered movie (81% of the differences in ΔF/F values are below 5%).
