Supplementary Figure 5: Granulomas form E-cadherin-positive adherens junctions that are maintained throughout Myco-GEM culture.
From: An explant technique for high-resolution imaging and manipulation of mycobacterial granulomas
(a) Granulomas were dissected from E-cadherin-tomato knock-in animals, placed in Myco-GEM culture conditions and immediately imaged for E-cadherin (magenta) or M. marinum (green) at the indicated depths. Yellow box indicates area magnified below. Scale bar, 50 µm. (b) After 5 d of Myco-GEM culture, granulomas were imaged for E-cadherin (magenta) or M. marinum (green), showing a similar organization of E-cadherin-positive adherens junctions after culture. Depth is indicated on images. For cultured granulomas, depths of images were chosen to match landmarks visualized in freshly dissected granulomas. Yellow box indicates magnified area below. Scale bar, 50 µm. Experiments in a,b were repeated twice with similar results each time.
