Supplementary Figure 6: Deep imaging within ex vivo–cultured granulomas by spinning disk confocal microscopy and light-sheet microscopy.
From: An explant technique for high-resolution imaging and manipulation of mycobacterial granulomas
Images of plakoglobin-citrine gene trap (yellow) and fluorescent M. marinum (cyan) at the indicated depths through the granuloma acquired by (a) spinning disk or (b) light-sheet microscopy. Granuloma images obtained ~ 1 h post-plating. Scale bars, 50 µm. Experiments in a were repeated independently 3 times and in b were repeated 2 times with similar results.
