Supplementary Figure 8: TNF transcriptional induction is maintained throughout granuloma culture.
From: An explant technique for high-resolution imaging and manipulation of mycobacterial granulomas
Granulomas from tnf:gfp animals infected with fluorescent M. marinum were cultured and longitudinally imaged for 5 d and tnf transcriptional induction was monitored by GFP expression, showing similar levels throughout culture. Gamma-adjusted images are shown to visualize GFP expression in the cells surrounding the necrotic core due to the substantial concentration of GFP within the necrotic core. GFP and M. marinum images are maximum projections of 70-µm z-stacks. Phase contrast images are single-plane images from near the center of the granuloma. TNF reporter experiments were independently repeated 3 times with similar results. Scale bar, 50 µm.
