Supplementary Figure 9: lux-expressing M. marinum enables quantitation of mycobacterial burden.
From: An explant technique for high-resolution imaging and manipulation of mycobacterial granulomas
(a) Images of cultured granulomas either untreated or treated with the indicated concentrations of anti-mycobacterial agents. Numbers under the images indicate the CFU count from plating and the relative light units (RLU) detected by plate reader. CFU results are representative from 3 granulomas from each group from a single experiment. (b) Graph of relative light units detected from untreated, 200 µg/ml isoniazid treated, or 80 µg/ml streptomycin treated granulomas at 3 days post-plating (dpp). Results are presented as mean ± s.d. for n = 9 granulomas. ****P < 0.0001 by one-way ANOVA with Tukey’s multiple comparison test. Luminescence results are representative of three independent experiments.
