Supplementary Figure 3: Bacteria remain under cellular stress during Myco-GEM culture.
From: An explant technique for high-resolution imaging and manipulation of mycobacterial granulomas
Mycobacterial stress was detected by HspX-driven GFP induction (green), while mycobacterial localization was detected by constitutive mCherry expression (red). High-contrast images demonstrating persistent stress within individual mycobacteria outside of the necrotic core. White arrowheads denote stressed mycobacteria that express both GFP and mCherry, magenta arrowheads denote unstressed mycobacteria expressing only mCherry. Lower contrast images demonstrating the stressed populations (expressing both GFP and mCherry) within the necrotic core of the granuloma. HspX reporter fluorescence was visualized in 3 independent experiments, with similar results in each experiment. Scale bar, 50 µm.
