Supplementary Figure 1: Reproducibility of photon count estimation. | Nature Methods

Supplementary Figure 1: Reproducibility of photon count estimation.

From: Impact of optical aberrations on axial position determination by photometry

Supplementary Figure 1

(A-C) Estimated photon counts and background photons per pixel for three measurements of three separated 45-nm-diameter beads imaged with an aberration-corrected microscope as a function of analysis area on the camera; pixel size, 80 nm. The three lines show the count for fitting with a fully fledged vectorial, TRABI or a simple Gaussian PSF.

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