Supplementary Figure 2: Comparison of photon count estimations in simulation and experiment.
From: Impact of optical aberrations on axial position determination by photometry
(A) Photon count estimation for a single simulated 45-nm bead PSF as a function of PSF image area on the camera for three different estimation algorithms: vectorial fit, TRABI, and Gaussian fit (8,750 signal photons, 15 background photons, same pixel size, NA and refractive index values as experiment). (B) The photometric ratio between Gaussian fit and TRABI photon count estimation as a function of PSF fit size. The dashed line indicates when the circular aperture size fits precisely within the square region of interest. (C-D) Same as panels (A,B) but for an experimentally recorded aberration-corrected 45-nm bead, indicating good quantitative agreement between experiment and simulation.
