Supplementary Figure 4: The effect of bead size on PSF shape.
From: Impact of optical aberrations on axial position determination by photometry
(A) The lateral and axial FWHM as a function of bead size. (B-C) Lateral average PSF in linear and log scale, respectively, as a function of the radial positon. The PSFs are displayed for different bead sizes. (D-E) On-axis PSF in linear and log scale, respectively, as a function of the axial position. The 45-nm bead captures the full tail behavior in both the lateral and axial directions of an actual single-molecule emitter, and has an FWHM within a few nanometers of a single-molecule emitter, whereas PSF details clearly get lost with larger (>90 nm) beads.
